Predicting Respiratory Toxicity Using a Human 3D Airway (EpiAirway™) Model Combined with Multiple Parametric Analysis
作者： Jamin A. Willoughby
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摘要: Abstract There is a growing need to develop, validate, and utilize new in vitro alternatives animal toxicity testing both chemical personal care industries. In the past, analysis of respiratory was performed using models; however, these studies are costly time-consuming. An human tissue model combined with multiple endpoint could provide robust for evaluating many types toxicity. The objective this study assess potential an three-dimensional airway (EpiAirway™) accurately predict humans. This done by exposing EpiAirway tissues known toxicants assessing viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT] assay histology), oxidative stress (cellular glutathione [GSH] levels), gene expression classical markers inflammation, metabolic activity, apoptosis quantitative real-time revers...
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