Lymphocytes and the foreign body response: lymphocyte enhancement of macrophage adhesion and fusion.

摘要: The host foreign body response ensues immediately following implantation of medical devices and prostheses. We have previously identified the role macrophages in adhering to biomaterial surfaces guiding while fusing into giant cells (FBGCs) concentrating degradative phagocytic activities. Despite their early transient presence around implanted biomaterials, few studies focused on lymphocytes biocompatibility. To address this, an vitro human lymphocyte/macrophage coculture system has been developed. Using this system, it shown that when are present during initial adhesion monocytes, rate monocyte fusion is significantly increased (1,500 cells/mm2 60%, respectively) compared either no (500 0% fusion). Although adhered tissue culture polystyrene surface, 90% were associated with adherent macrophages. However, these cell-cell direct interactions not necessary influence macrophage or as separating two cell types by a Transwell insert still resulted levels (p < 0.05 only cultures). Conversely, experiments lymphocyte proliferation rates at all time points tested. These begin detail between absence known antigen appropriately relates scenarios experienced upon biomedical initiation response.