To study the effect of curcumin on the growth properties of circulating endothelial progenitor cells

摘要: Curcumin has been shown to regulate the expression of genes implicated in tumor cell proliferation, metastasis, chemotherapy resistance, and angiogenesis. Endothelial progenitor cells (EPCs) have recently described peripheral blood as contributing both physiological pathological In current study, we evaluated effect curcumin on these angiogenic cells. EPCs were isolated, expanded, characterized ex vivo. These then treated with different concentrations curcumin. The formation EPC colonies culture their proliferation was analyzed by 5′-bromo-2′-deoxyuridine assays absence presence Further, two important cycle inhibitory proteins, p21 p53, curcumin- medium-treated without intracellular flow cytometry. results showed that there a significant decrease culture. significantly inhibited dose-dependent manner. Flow cytometry analysis twofold increase p53 curcumin-treated compared cells, suggesting inhibits growth mainly inhibiting G1 S phase transition cycle. It would be further worthwhile study EPC-mediated activity.