Purification of d-hydantoinase from adzuki bean and its immobilization for N-carbamoyl-d-phenylglycine production
作者： Chia-Hsi Fan , Cheng-Kang Lee
DOI: 10.1016/S1369-703X(01)00098-5
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摘要: Abstract Hydantoinase could be extracted from adzuki bean by a simple separation process. The molecular weight of the partially purified hydantoinase determined MALDI-TOF mass spectrometry was 52.5 kDa. enzyme to d -specific and preferred substrate -phenylhydantoin (PH) rather than - p -hydroxy-phenylhydantoin ( HPH). Its specific activity towards PH about sixfold that HPH. retained 76% its after incubation at 40°C for 6 days. immobilization easily achieved mixing solution with fine polyglutaraldehyde (PGL) particles -hydantoinase transform 1% (w/v) into N -carbamoyl- -phenylglycine -CPG) >95% conversion very good stability no appreciable loss observed five repeated batch reactions 40°C.
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