Purification and characterization of bacteriophage N4-induced DNA polymerase.
作者： G K Lindberg , J K Rist , T A Kunkel , A Sugino , L B Rothman-Denes
DOI: 10.1016/S0021-9258(18)37961-4
关键词:
摘要: Coliphage N4 replication is independent of most host DNA functions except for the 5'----3' exonuclease activity polA, ligase, gyrase, and ribonucleotide reductase (Guinta, D., Stambouly, J., Falco, S. C., Rist, J. K., Rothman-Denes, L. B. (1986) Virology 150, 33-44). It therefore expected that codes required its genome. In this paper we report purification N4-coded polymerase from N4-infected cell extracts by following on a gapped template in an vitro complementation system (Rist, Pearle, M., Sugino, A., Biol. Chem. 261, 10506-10510). The enzyme composed one polypeptide, Mr 87,000. active templates containing short gaps synthesizing with high fidelity quasi-processive manner. A strong 3'----5' associated polypeptide. No or strand-displacing activities were detected.
sci-hub.st 参考文章(42)
P Modrich, C C Richardson, Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. Journal of Biological Chemistry. ,vol. 250, pp. 5515- 5522 ,(1975) , 10.1016/S0021-9258(19)41212-X
Nicholas Muzyczka, Ronald L. Poland, Maurice J. Bessman, Studies on the Biochemical Basis of Spontaneous Mutation Journal of Biological Chemistry. ,vol. 247, pp. 7116- 7122 ,(1972) , 10.1016/S0021-9258(19)44602-4
J K Rist, M Pearle, A Sugino, L B Rothman-Denes, Development of an in vitro bacteriophage N4 DNA replication system. Journal of Biological Chemistry. ,vol. 261, pp. 10506- 10510 ,(1986) , 10.1016/S0021-9258(18)67413-7
D Guinta, G Lindberg, L B Rothman-Denes, Bacteriophage N4-coded 5'----3' exonuclease. Purification and characterization. Journal of Biological Chemistry. ,vol. 261, pp. 10736- 10743 ,(1986) , 10.1016/S0021-9258(18)67447-2
A.C. Roth, N.G. Nossal, P.T. Englund, Rapid hydrolysis of deoxynucleoside triphosphates accompanies DNA synthesis by T4 DNA polymerase and T4 accessory proteins 44/62 and 45. Journal of Biological Chemistry. ,vol. 257, pp. 1267- 1273 ,(1982) , 10.1016/S0021-9258(19)68186-X
R. Zivin, W. Zehring, L.B. Rothman-Denes, Transcriptional map of bacteriophage N4: Location and polarity of N4 RNAs Journal of Molecular Biology. ,vol. 152, pp. 335- 356 ,(1981) , 10.1016/0022-2836(81)90246-1
W A Zehring, L B Rothman-Denes, Purification and characterization of coliphage N4 RNA polymerase II activity from infected cell extracts. Journal of Biological Chemistry. ,vol. 258, pp. 8074- 8080 ,(1983) , 10.1016/S0021-9258(20)82029-8
C.S. McHenry, W. Crow, DNA polymerase III of Escherichia coli. Purification and identification of subunits. Journal of Biological Chemistry. ,vol. 254, pp. 1748- 1753 ,(1979) , 10.1016/S0021-9258(17)37836-5
T A Kunkel, P S Alexander, The base substitution fidelity of eucaryotic DNA polymerases. Mispairing frequencies, site preferences, insertion preferences, and base substitution by dislocation. Journal of Biological Chemistry. ,vol. 261, pp. 160- 166 ,(1986) , 10.1016/S0021-9258(17)42446-X
S.C. Falco, W. Zehring, L.B. Rothman-Denes, DNA-dependent RNA polymerase from bacteriophage N4 virions. Purification and characterization. Journal of Biological Chemistry. ,vol. 255, pp. 4339- 4347 ,(1980) , 10.1016/S0021-9258(19)85670-3