HIV Rev Assembly on the Rev Response Element (RRE): A Structural Perspective

摘要: HIV-1 Rev is an ~13 kD accessory protein expressed during the early stage of virus replication. After translation, enters nucleus and binds response element (RRE), a ~350 nucleotide, highly structured embedded in env gene unspliced singly spliced viral RNA transcripts. Rev-RNA assemblies subsequently recruit Crm1 other cellular proteins to form larger complexes that are exported from nucleus. Once cytoplasm, dissociate singly-spliced RNAs packaged into nascent virions or translated structural enzymes, respectively. binding RRE complex process, as multiple copies assemble on coordinated fashion via series Rev-Rev interactions. Our understanding nature these interactions has been greatly advanced by recent studies using X-ray crystallography, small angle scattering (SAXS) single particle electron microscopy well biochemical genetic methodologies. These advances discussed detail this review, along with perspectives development antiviral therapies targeting RRE.