K-ras point mutations in human colorectal carcinomas: relation to aneuploidy and metastasis.

摘要: Material from paraffin sections of 109 human colorectal carcinomas, mostly obtained at autopsy, was analyzed for the presence K-ras point mutations codon 12, position 2. Mutations this were found in 23 cases (21.1%). Aneuploid carcinomas showed a significantly higher prevalence than diploid tumors, suggesting an involvement ras development aneuploidy. No differences observed with respect to patients' age, sex and tumor type. In metastases, type gene mutation always identical that respective primary tumor. not metastases tumors devoid mutations. This renders clonal selection mutated cells wild-type during metastatic process unlikely. However, nearly twice as many seen non-metastatic tumors. © 1992 Wiley-Liss, Inc.