Temporal regulation of human cytomegalovirus transcription at immediate early and early times after infection.

摘要: The immediate early transcripts of human cytomegalovirus originated from restricted regions the viral genome. In contrast, transcription at times was complementary to all genome that were fractionated by restriction endonuclease treatment followed agarose gel electrophoresis. also extensively transcribed when 2 h protein synthesis or longer permitted after infection in permissive cells treated with an inhibitor DNA replication nonpermissive animal origin permit little no replication. size and vitro translation products cytomegalovirus-specified mRNA's determined. Discrete classes virus-specified polyadenylated RNA accumulated on polyribosomes infected presence synthesis. When 24 occurred infection, there changes relative abundance RNAs polyribosomes. Treatment had effect found associated infection. These assumed represent gene expression. mRNA isolated identified many demonstrated (i) a switch expression (ii) prolonged phase data indicated initiation does not depend formation protein, but de novo may influence extent