Nano-titanium dioxide composites for the enrichment of phosphopeptides.

摘要: Abstract Protein phosphorylation is one of the most important known posttranslational modifications and strategy to enrich phosphopeptides becomes a critical issue for mapping protein sites. In this study, nano-titanium dioxide (TiO2) composites were synthesized, characterized, demonstrated have high loading capacity capture efficiency enriching phosphopeptides. TiO2 nanoparticles first silanized with methacryloxypropyltrimethoxysilane (MPTMS) then photopolymerized in presence diacrylate crosslinker. The chemical bonds formed by reaction confirmed both FT-IR X-ray photoelectron spectroscopy (XPS). Scanning electron microscopy (SEM) further reveals that agglomeration particles was created crosslinking, which allowed nanocomposites be well retained within cartridge used as chromatographic packing material. Titration phenyl phosphate indicated two times much binding compared 5 μm particles. Moreover, based on repetitive analyses tryptic digest deduced from pure proteins mixtures containing phospho non-phospho proteins, determined five larger cost preparing nanocomposite low it holds great promises materials phosphopeptide enrichment.