Etude de la régulation de deux protéines kinases : GSK-3 et polo
作者： Aline Primot
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摘要: Nous avons etudie 2 proteines kinases : GSK-3 et Polo. La "Glycogen Synthase Kinase-3" intervient dans divers processus cellulaires. demontre que peut etre purifiee par chromatographie d'affinite sur axine fixee des billes de seraphose. clone le gene d'une proteine homologue humaine chez parasite responsable du paludisme. PfGSK-3 est localisee au niveau la membrane plasmique l'erythrocyte a l'interieur parasite. L'utilisation comme cible devrait permettre decouverte d'inhibiteurs selectifs dont les effets therapeutiques potentiels seront evalues. Polo differents niveaux regulation cellulaire. Le clonage partie l'ADNc d'oursin (Plu1) permis synthese d'anticorps specifiques. L'immunoprecipitation Plu1 cours premieres divisions d'embryon revele se lie differentes cycle l'identite reste confirmer.
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