Expression of the Oligodendroglial Lineage-Associated Markers Olig1 and Olig2 in Different Types of Human Gliomas

摘要: Because a specific group of oligodendrogliomas is susceptible to adjuvant therapy, it important elucidate the biological characteristics these tumors. In situ hybridization analyses have revealed that Olig genes are expressed in oligodendroglial lineage cells and highly oligodendrogliomas. To clarify whether OLIG tumor-specific marker for oligodendrogliomas, we investigated expression transcripts by semiquantitative RT-PCR assay OLIG2 protein with new antibody variety glial The high levels Olig1 Olig2 mRNAs were present anaplastic astrocytomas, while grade IV glioblastomas was lower than II III gliomas (p < 0.01). Immunohistochemical demonstrated mean immunopositive proportion 82% but only 34% astrocytomas. Therefore, although detected range not level more uniform intense other conclusion, combining mRNA immunohistochemistry enables be distinguished from astrocytic