Adsorption of glucose oxidase onto single-walled carbon nanotubes and its application in layer-by-layer biosensors.
作者： Ta-Wei Tsai , Gustavo Heckert , Luís F. Neves , Yongqiang Tan , Der-You Kao
DOI: 10.1021/AC900650R
关键词:
摘要: In this study, we describe the use of a sodium cholate suspension-dialysis method to adsorb redox enzyme glucose oxidase (GOX) onto single-walled carbon nanotubes (SWNT). By method, solutions dispersed and debundled SWNTs were prepared that remained stable for 30 days which retained 75% native enzymatic activity. We also demonstrate GOX-SWNT conjugates can be assembled into amperometric biosensors with poly[(vinylpyridine)Os(bipyridyl)2Cl2+/3+] polymer (PVP-Os) through layer-by-layer (LBL) self-assembly process. Incorporation SWNT-enzyme LBL films resulted in current densities as high 440 μA/cm2, 2-fold increase over response without SWNTs. adsorption pH solution dispersion quality important parameters controlling electrochemical properties films.
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