Functionalized carbon nanotubes for detecting viral proteins

摘要: We investigated the biocompatibility, specificity, and activity of a ligand−receptor-protein system covalently bound to oxidized single-walled carbon nanotubes (SWNTs) as model proof-of-concept for employing such SWNTs biosensors. were functionalized under ambient conditions with either Knob protein domain from adenovirus serotype 12 (Ad Knob) or its human cellular receptor, CAR protein, via diimide-activated amidation. confirmed biological immobilized on nanotube surfaces by using labeled conjugate antibody evaluated specificity SWNTs, first, in presence fluorescently Knob, which interacts specifically CAR, second, negative control YieF, is not recognized biologically active proteins. In addition, current−gate voltage (I−Vg) measurements dozen devices explored effect binding intrinsic electronic properties SWNTs,...