Antisuppressor mutations and sulfur-carrying nucleosides in transfer RNAs of Schizosaccharomyces pombe.
作者： A M Grossenbacher , B Stadelmann , W D Heyer , P Thuriaux , J Kohli
DOI: 10.1016/S0021-9258(18)66573-1
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摘要: Antisuppressor mutations reduce the efficiency of nonsense suppressors. A mutation in gene sin4 Schizosaccharomyces pombe leads to loss 5-(methoxycarbonylmethyl) thiouridine (mcm5s2U) from first anticodon position tRNAs. This resembles phenotype sin3 (Heyer, W. D., Thuriaux, P., Kohli, J., Ebert, Kersten, H., Gehrke, C., Kuo, K. and Agris, P. F. (1984) J. Biol. Chem. 259, 2856-2862), but reside different genes. In vivo 35S-labeled tRNA parental suppressor strain sup3, antisuppressor strains sin4, double mutant has been digested nucleosides analyzed with high performance liquid chromatography methods. The major sulfur-carrying nucleoside wild-type S. is mcm5s2U. It reduced strains. Two other thiolated are also present: 2-thiouridine a unknown structure. Neither was affected by mutations. Thiocytidine not found. Independent their effect on suppressors, two growth rate cells, increases cell length. decoding serine codon UCG UCA reading promoted
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sci-hub.st 参考文章(12)
P. Thuriaux, M. Minet, F. Hofer, U. Leupold, Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe. Molecular Genetics and Genomics. ,vol. 142, pp. 251- 261 ,(1975) , 10.1007/BF00271250
S Patwardhan, J D Cherayil, 5-Methyl-2-thiouridine in the tRNA of Candida tropicalis and its localization in lysine tRNA. Journal of Bacteriology. ,vol. 162, pp. 55- 60 ,(1985) , 10.1128/JB.162.1.55-60.1985
Charles W. Gehrke, Kenneth C. Kuo, Roy A. McCune, Klaus O. Gerhardt, Paul F. Agris, Quantitative enzymatic hydrolysis of tRNAs Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 230, pp. 297- 308 ,(1982) , 10.1016/S0378-4347(00)80479-X
W D Heyer, P Thuriaux, J Kohli, P Ebert, H Kersten, C Gehrke, K C Kuo, P F Agris, An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the “wobble” base in the anticodon of tRNAs. Journal of Biological Chemistry. ,vol. 259, pp. 2856- 2862 ,(1984) , 10.1016/S0021-9258(17)43226-1
Peter Munz, Hanspeter Amstutz, Jürg Kohli, Urs Leupold, Recombination between dispersed serine tRNA genes in Schizosaccharomyces pombe Nature. ,vol. 300, pp. 225- 231 ,(1982) , 10.1038/300225A0
Robert W. Zumwalt, Kenneth C. T. Kuo, Paul F. Agris, Melanie Ehrlich, Charles W. Gehrke, High Performance Liquid Chromatography of Nucleosides in RNA and DNA Journal of Liquid Chromatography & Related Technologies. ,vol. 5, pp. 2041- 2060 ,(1982) , 10.1080/01483918208067616
N. Shaun B. Thomas, Philip D. Butcher, Henry R.V. Arnstein, Polyribosome binding of rabbit globin messenger RNA and messenger ribonucleoprotein labelled with bacteriophage-T4 RNA ligase and 5'-[32P] phosphocytidine 3'-phosphate. Nucleic Acids Research. ,vol. 11, pp. 1- 10 ,(1983) , 10.1093/NAR/11.1.1
Howard M. Laten, Jane Harris Cramer, Robert H. Rownd, Thiolated nucleotides in yeast transfer RNA Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. ,vol. 741, pp. 1- 6 ,(1983) , 10.1016/0167-4781(83)90002-7
Charles W. Gehrke, Kenneth C. Kuo, George E. Davis, Robert D. Suits, T.Phillip Waalkes, Ernest Borek, Quantitative high-performance liquid chromatography of nucleosides in biological materials Journal of Chromatography A. ,vol. 150, pp. 455- 476 ,(1978) , 10.1016/S0021-9673(00)88205-9
F. Janner, G. Vögeli, R. Fluri, The antisuppressor strain sin1 of Schizosaccharomyces pombe lacks the modification isopentenyladenosine in transfer RNA. Journal of Molecular Biology. ,vol. 139, pp. 207- 219 ,(1980) , 10.1016/0022-2836(80)90305-8