Electrically-enhanced microextraction combined with maltodextrin-modified capillary electrophoresis for quantification of tolterodine enantiomers in biological samples

摘要: Abstract In this work, a recent novel electrically-enhanced microextraction technique, termed electromembrane extraction (EME) combined with maltodextrin-modified capillary electrophoresis (CE) was used for separation, preconcentration and determination of tolterodine (TOL) enantiomers in biological fluids. During the extraction, TOL migrated from 3 mL sample solution through supported liquid membrane (SLM) consisting 2-nitrophenyl octyl ether (NPOE) immobilized pores hollow fiber, into 20 μL acidic aqueous acceptor presented inside lumen fiber. A fractional factorial design (FFD) employed screening to determine variables significantly affecting efficiency. Then, significant factors were optimized by using Box–Behnken (BBD) response surface methodology (RSM). Optimized conditions were: NPOE as SLM, stirring rate 1200 rpm, 54 V potential difference, 24 min time, phase (500 mM HCl) donor (10 mM HCl). extract analyzed maltodextrin (MD) modified CE method separation enantiomers. Best results achieved 100 mM phosphate running buffer (pH 4.0) containing 20% (w/v) MD dextrose equivalent (DE) 4–7 chiral selector, applied voltage 20 kV 20 °C. The reproducible so that intra inter day RSDs (n = 5) less than 6.5%. limits quantitation detection both 10 3 ng mL− 1, respectively. Finally, proposed successfully concentration plasma urine samples.