Mechanisms of phytohaemagglutinin (PHA) stimulation of normal human lymphocytes: ‘trigger’, ‘push’ or both?
作者： J. H. Gibbs , R. C. Potts , R. A. Brown , A. J. Robertson , J. Swanson Beck
DOI: 10.1111/J.1365-2184.1982.TB01031.X
关键词:
摘要: Abstract. The growth in volume of human peripheral blood lymphocytes after stimulation with various concentrations PHA was measured an electronic particle counter. percentage growing cells and averaged values describing their rates during the elapsed period culture were estimated by fitting to observed data distributions derived from a mathematical model. With sub-optimal doses, stimulated, incremental rate, increased increasing dose PHA, but time-course recruitment into G1-phase similar all studied. results provide strong support for ‘trigger’ hypothesis that there is distribution thresholds within lymphocyte population: consequently, mitogen concentration will be expected result numbers responding suboptimal range.
sci-hub.se 参考文章(9)
Linda H. Younkin, In vitro response of lymphocytes to phytohemagglutinin (PHA) as studied with antiserum to PHA. II. Cell cycle analyses. Experimental Cell Research. ,vol. 90, pp. 374- 380 ,(1975) , 10.1016/0014-4827(75)90327-4
Janina Jasińska, J.A. Steffen, A. Michałowski, Studies on in vitro lymphocyte proliferation in cultures synchronized by the inhibition of DNA synthesis: II. Kinetics of the initiation of the proliferative response Experimental Cell Research. ,vol. 61, pp. 333- 341 ,(1970) , 10.1016/0014-4827(70)90455-6
N. R. Ling, P. J. L. Holt, The activation and reactivation of peripheral lymphocytes in culture. Journal of Cell Science. ,vol. 2, pp. 57- 70 ,(1967)
C. E. Mogensen, The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 21, pp. 77- 82 ,(1968) , 10.3109/00365516809076979
R.A. Brown, J.H. Gibbs, A.J. Robertson, R.C. Potts, J.Swanson Beck, Development of asynchrony in growth of normal human lymphocytes during first day of culture after PHA stimulation. Experimental Cell Research. ,vol. 126, pp. 87- 97 ,(1980) , 10.1016/0014-4827(80)90473-5
R.C. Potts, J.H. Gibbs, A.J. Robertson, R.A. Brown, J. Swanson Beck, A simple method for determining the extent of cellular contamination in peripheral blood lymphocyte preparations Journal of Immunological Methods. ,vol. 35, pp. 177- 187 ,(1980) , 10.1016/0022-1759(80)90245-8
J.H. Gibbs, R.A. Brown, A.J. Robertson, R.C. Potts, J. Swanson Beck, A new method of testing for mitogen-induced lymphocyte stimulation: Measurement of the percentage of growing cells and of some aspects of their cell kinetics with an electronic particle counter Journal of Immunological Methods. ,vol. 25, pp. 147- 158 ,(1979) , 10.1016/0022-1759(79)90050-4
R. A. Brown, R. Mcwalter, W. Slidders, J. Gibbs, J. Swanson Beck, Measurement by Quantimet 720 of the proportion of actively growing cells in tissue cultures of human lymphocytes Journal of Microscopy. ,vol. 115, pp. 51- 63 ,(1979) , 10.1111/J.1365-2818.1979.TB00151.X
A. Boyum, Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 21, pp. 77- 89 ,(1968)