Electron Cryomicroscopy of E. coli Reveals Filament Bundles Involved in Plasmid DNA Segregation

摘要: Bipolar elongation of filaments the bacterial actin homolog ParM drives movement newly replicated plasmid DNA to opposite poles a cell. We used combination vitreous sectioning and electron cryotomography study this partitioning system directly in native, frozen cells. The diffraction patterns from overexpressed bundles cryotomographic reconstructions were unambiguously identify Escherichia coli Using low-copy number encoding components required for partitioning, we observed small three five intracellular that situated close edge nucleoid. propose may indicate capture within periphery loosely defined, chromosome-containing region.