Splicing Variants of SERPINA1 Gene in Ovine Milk: Characterization of cDNA and Identification of Polymorphisms

摘要: The serine protease inhibitor, clade A, member 1 (SERPINA1) is the gene for a protein called alpha-1-antitrypsin (AAT), which of inhibitor (serpin) superfamily proteins. By conformational change, serpins control several chemical reactions inhibiting activity proteases. AAT most abundant endogenous serpin in blood circulation and it present relatively high concentration human milk as well bovine porcine colostrum. Here we report first time molecular characterization sequence variability ovine SERPINA1 cDNA gene. cDNAs from mammary gland were PCR amplified, three different transcripts (1437, 1166 521bp) identified. We amplified sequenced regions (5’ UTR, exon 2 to 5 3’ UTR), found that exon-intron structure similar bovine. detected total 97 SNPs sequences 10 sheep breeds. In adult tissues expression analysis indicated differential transcripts. finding reported this paper will aid further studies on possible involvement physiological states its association with production traits.