DNA methylation map in circulating leukocytes mirrors subcutaneous adipose tissue methylation pattern: a genome-wide analysis from non-obese and obese patients

作者: J. A. Martinez , A. B. Crujeiras , A. Diaz-Lagares , J. Sandoval , F. I. Milagro

DOI: 10.1038/SREP41903

关键词:

摘要: The characterization of the epigenetic changes within obesity-related adipose tissue will provide new insights to understand this metabolic disorder, but is not easy sample in population-based studies. We aimed evaluate capacity circulating leukocytes reflect tissue-specific DNA methylation status obesity susceptibility. samples isolated from subcutaneous and were hybridized Infinium HumanMethylation 450 BeadChip. Data compared between obese (n = 45) non-obese (n = 8-10) patients by Wilcoxon-rank test, unadjusted for cell type distributions. A global hypomethylation differentially methylated CpG sites (DMCpGs) was observed leukocytes. overlap analysis yielded a number genes mapped common DMCpGs that identified state Specifically, levels FGFRL1, NCAPH2, PNKD SMAD3 exhibited excellent statistically significant efficiencies discrimination non-obesity (AUC > 0.80; p < 0.05) great correlation both tissues. Therefore, current study provided valuable biomarkers pathogenesis through peripheral blood analysis, an easily accessible minimally invasive biological material instead tissue.

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