Microbial aldolases as C–C bonding enzymes—unknown treasures and new developments

摘要: Aldolases are a specific group of lyases that catalyze the reversible stereoselective addition donor compound (nucleophile) onto an acceptor (electrophile). Whereas most aldolases for their in aldolization reaction, they often tolerate wide range aldehydes as compounds. C–C bonding by creates stereocenters resulting aldol products. This makes interesting tools asymmetric syntheses rare sugars or sugar-derived compounds iminocyclitols, statins, epothilones, and sialic acids. Besides well-known fructose 1,6-bisphosphate aldolase, other microbial origin have attracted interest synthetic bio-organic chemists recent years. These either dihydroxyacetone phosphate depending on pyruvate/phosphoenolpyruvate, glycine, acetaldehyde substrate. Recently, aldolase accepts hydroxyacetone was described. A further enlargement arsenal available chemoenzymatic can be achieved through screening novel activities directed evolution existing to alter substrate- stereospecifities. We give update work aldolases, with emphasis aldolases.