PIK3CA Mutations May Be Discordant between Primary and Corresponding Metastatic Disease in Breast Cancer

摘要: Purpose: PIK3CA mutations are frequent in breast cancer and activate the PI3K/Akt pathway. Unexpectedly, mutation appears general to be associated with better outcome. In a cohort of patients where both primary metastatic lesions were available, objective was assess changes mutations. We wished discern whether selective pressures occur influence on time recurrence. Experimental Design: Formalin-fixed paraffin-embedded tumor blocks obtained from 104 paired samples tumors corresponding asynchronous tumors. Samples analyzed for (exons 9 20) as well immunohistochemical evaluation PTEN, pAKT, Ki67, ER, HER2. Results: detected 45% Overall, there net gain disease, 53%; nonetheless, instances metastases wild type mutant Laser capture microdissection subset cases revealed microheterogeneity mutational status tumor. mutants overall showed significantly longer first recurrence than ( P = 0.03). Conclusion: at high frequency cancer; these may not necessarily confer increased aggressiveness had Because quite frequently between it emphasizes necessity assessing lesion selection inhibitor therapy. Clin Cancer Res; 17(4); 667–77. ©2010 AACR.