New fusion protein systems designed to give soluble expression in Escherichia coli.
作者： Gregory D. Davis , Claude Elisee , Denton M. Newham , Roger G. Harrison
DOI: 10.1002/(SICI)1097-0290(19991120)65:4<382::AID-BIT2>3.0.CO;2-I
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摘要: … washed to baseline with loading buffer (50 mM Tris-… SDS–PAGE and Western blot results (Fig. 2) that the vast majority of the NusA/bGH fusion protein is expressed in the soluble fraction …
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