Identification of a second human nm23 gene, nm23-H2.
作者： P. S. Steeg , J. A. Stahl , A. M. Rosengard , A. Leone , L. Porter
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摘要: Reduced RNA and/or protein levels corresponding to the murine nm23-1 and human nm23-H1 complementary DNA clones have been correlated with high tumor metastatic potential in several rodent model systems breast carcinomas. We report identification of a second nm23 gene, designated nm23-H2. The pNM23-H2S clone predicted Mr 17,000 88% identical nm23-H1. nm23-H2 also shared significant homology nucleoside diphosphate kinases Drosophila developmental gene. Southern blots containing BglII-restricted genomic DNA, which exhibited an allelic restriction fragment length polymorphism for nm23-H1, contained nonallelic bands upon rehybridization probe. Thus, are distinct genes. Northern blot hybridization nm23-H1- nm23-H2-specific probes tumors cell lines indicated that expression was reduced cells greater extent than data indicate existence family independently regulated
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