Experimental therapy of prostate cancer with novel natural product anti-cancer ginsenosides

摘要: BACKGROUND Ginseng and its components exert various biological effects, including antioxidant, anti-carcinogenic, anti-mutagenic, anti-tumor activity, recent research has focused on their value in human cancer prevention treatment. We recently isolated 25-hydroxyprotopanaxadiol (25-OH-PPD) 25-hydroxyprotopanaxatriol (25-OH-PPT) from Panax ginseng evaluated anti-cancer activity vitro. METHODS We compared the effects of two compounds prostate LNCaP PC3 cells vitro a mouse xenograft tumor model. also accomplished preliminary determination mechanisms action compounds. RESULTS 25-OH-PPD, but not 25-OH-PPT, inhibited cell growth proliferation, induced apoptosis, led to arrest G1 phase cycle. In nude mice bearing tumors, 25-OH-PPD dose-dependent manner could be safely combined with chemotherapeutic agents (taxotere gemcitabine) radiation therapy improve effects. Further, both lines, increased expression p21, p27, Bax, PARP cleavage activated caspases. The compound reduced MDM2, E2F1, Bcl2, cdk2/4/6, cyclin D1, which correlated cycle decrease proliferation. Moreover, demonstrated low toxicity non-cancer no observable host animals either alone or combination conventional therapies. CONCLUSIONS The newly identified ginsenoside may have potential as novel therapeutic agent. Prostate 68:809–819, 2008. © 2008 Wiley-Liss, Inc.