The unconventional kinetoplastid kinetochore: from discovery toward functional understanding.

摘要: The kinetochore is the macromolecular protein complex that drives chromosome segregation in eukaryotes. Its most fundamental function to connect centromeric DNA dynamic spindle microtubules. Studies popular model eukaryotes have shown centromere (CENP)-A critical for DNA-binding, whereas Ndc80 essential microtubule-binding. Given their conservation diverse eukaryotes, it was widely believed all would utilize these components make up a core of kinetochore. However, recent study identified an unconventional type evolutionarily distant kinetoplastid species, showing can be achieved using distinct set proteins. Here, I review discovery two systems and discuss how studies contribute better understanding eukaryotic machinery.