IFN-gamma negatively modulates self-renewal of repopulating human hemopoietic stem cells.

摘要: Whereas multiple growth-promoting cytokines have been demonstrated to be involved in regulation of the hemopoietic stem cell (HSC) pool, potential role negative regulators is less clear. However, IFN-gamma, if overexpressed, can mediate bone marrow suppression and has directly implicated a number failure syndromes, including graft-vs-host disease. Whether IFN-gamma might affect function repopulating HSCs has, however, not investigated. In present study, we used vitro conditions promoting self-renewing divisions human investigate effect on HSC maintenance function. Although purified cord blood CD34(+)CD38(-) cells underwent presence cycling exposed were severely compromised their ability reconstitute long-term cultures multilineage engraft NOD-SCID mice vivo (>90% reduced activity both assays). studies suggested that accelerated differentiation targeted progenitor cells. These results demonstrate negatively self-renewal.