Efficient species identification of pine marten ( Martes martes ) and red fox ( Vulpes vulpes ) scats using a 5′ nuclease real-time PCR assay
作者： Catherine O’Reilly , Mark Statham , Jacinta Mullins , Peter D. Turner , Declan O’Mahony
DOI: 10.1007/S10592-007-9371-6
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摘要: Monitoring wildlife species by DNA identification of samples collected non-invasively is an important tool in conservation management. from faecal (scat) problematic due to the small quantities and poor quality isolated such samples. This study demonstrates use real-time PCR technology red fox (Vulpes vulpes) pine marten (Martes martes). It shown that can be used identify either melting curve analysis (Tm determination) with SYBR Green 1 detection or specific fluorogenic probes. The technique work efficiently scat DNA.
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Huw I. Griffiths, Angus Davison, Johnny D. S. Birks, Rachael C. Brookes, John E. Messenger, Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Molecular Ecology. ,vol. 10, pp. 2479- 2488 ,(2002) , 10.1046/J.1365-294X.2001.01381.X
K J Livak, S J Flood, J Marmaro, W Giusti, K Deetz, Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. Genome Research. ,vol. 4, pp. 357- 362 ,(1995) , 10.1101/GR.4.6.357
M. Statham, C. O'Reilly, P. D. Turner, Use of PCR amplification and restriction enzyme digestion of mitochondrial D-loop for identification of mustelids in Ireland ,(2005)
M. M. Hansen, L. Jacobsen, Identification of mustelid species: otter (Lutra lutra), American mink (Mustela vison) and polecat (Mustela putorius), by analysis of DNA from faecal samples Journal of Zoology. ,vol. 247, pp. 177- 181 ,(1999) , 10.1111/J.1469-7998.1999.TB00981.X
Angus Davison, Johnny D. S. Birks, Rachael C. Brookes, Tony C. Braithwaite, John E. Messenger, On the origin of faeces: morphological versus molecular methods for surveying rare carnivores from their scats Journal of Zoology. ,vol. 257, pp. 141- 143 ,(2002) , 10.1017/S0952836902000730