Akt/PKB regulates laminin and collagen IV isotypes of the basement membrane

摘要: Basement membranes are important for epithelial differentiation, cell survival, and normal metastatic migration. Much is known about their breakdown remodeling, yet positive regulation poorly understood. Our previous analysis of a fibroblast growth factor (FGF) receptor mutation raised the possibility that protein kinase B (Akt/PKB) activated by FGF connected to expression certain laminin type IV collagen isotypes. Here we test this hypothesis demonstrate constitutively active Akt/PKB, an downstream element phosphoinositide 3′-kinase signaling, induces synthesis laminin-1 isotypes causes translocation basement membrane. By using promoter–reporter constructs, show 3′-kinase-p110 or Akt/PKB activates, whereas dominant negative inhibits, transcription β1 α1 in differentiating C2 myoblast- insulin-induced Chinese hamster ovary–T cultures. These results suggest tyrosine kinases involved membrane formation. The possible role Akt/PKB-induced survival differentiation will be discussed.