Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani.
作者： Daniel Sevlever , Peter P�hlsson , Graciela Rosen , Bo Nilsson , Mauricio V. Londner
DOI: 10.1007/BF00731344
关键词:
摘要: A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated fromLeishmania donovani promastigotes. The carbohydrate moiety cleaved the lipid part digestion specific phosphatidylinositol phospholipase C. After separation, structural analysis carried out on phosphorylated inositol oligosaccharide and alkylacyl glycerol. following major structures were found: $$\begin{gathered} Man\alpha 1 - 2Man\alpha \hfill \\ \begin{array}{*{20}c} \backslash 6 { } 3 {Man\alpha 1/} \end{array} 4GlcN\alpha O \parallel P | {O^ _{\begin{array}{*{20}c} {CH_2 {CH} {{\text{ CO (CH}}_{\text{2}} {\text{)}}_n {\text{ CH}}_{\text{3}} {\text{)}}_m n = 16,18,20,22,24,26 m 16,17,18,21 \end{gathered} $$ presence of conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI glycosyl protein anchors in this may be consistent a precursor role ofLeishmania anchored proteins for glycolipid.
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