Non-linear kinetics of glutamyl-tRNA synthesis catalyzed by high molecular weight complexes from rat brain neuronal cells but not from glial cells.
作者： Lucille Lacoste , Leif Hertz , Jacques Lapointe
DOI: 10.1016/0169-328X(86)90016-1
关键词:
摘要: Abstract High molecular weight complexes of aminoacyl-tRNA synthetases isolated from rat brain catalyze the formation glutamyl-tRNA with an initial lag time order 1 min, as previously reported for and glutaminyl-tRNA catalyzed by similar bovine (Vadeboncoeur Lapointe, Eur. J. Biochem., 109 (1980) 581–587). To determine type(s) cell(s) where this phenomenon occurs, we have studied kinetics high neuronal glial cells, either transformed (Neuro-2A C6), or primary cultures, brain. The delay in was observed only case cells brain, whereas a also seen these well culture synaptosomes. aspartyl-tRNA valyl-tRNA all linear.
elsevier.com
sci-hub.se 参考文章(24)
Arnold E. Hampel, M. Duane Enger, Preston O. Ritter, [17] Aminoacyl-tRNA synthetases from cultured CHO cells Methods in Enzymology. ,vol. 59, pp. 229- 234 ,(1979) , 10.1016/0076-6879(79)59086-7
Booher J, Sensenbrenner M, Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology. ,vol. 2, pp. 97- 105 ,(1972)
V. P. Whittaker, E. G. Gray, The isolation of nerve endings from brain: An electron microscopic study of cell fragments derived by homogenization and centrifugation Journal of Anatomy. ,vol. 96, pp. 79- 88 ,(1962)
Arne Schousboe, Transport and Metabolism of Glutamate and Gaba in Neurons and Glial Cells International Review of Neurobiology. ,vol. 22, pp. 1- 45 ,(1981) , 10.1016/S0074-7742(08)60289-5
Shirley E. Poduslo, William T. Norton, [54] Isolation of specific brain cells Methods in Enzymology. ,vol. 35, pp. 561- 579 ,(1975) , 10.1016/0076-6879(75)35185-9
Carl Frieden, Kinetic Aspects of Regulation of Metabolic Processes: THE HYSTERETIC ENZYME CONCEPT Journal of Biological Chemistry. ,vol. 245, pp. 5788- 5799 ,(1970) , 10.1016/S0021-9258(18)62721-8
O Kellermann, H Tonetti, A Brevet, M Mirande, J P Pailliez, J P Waller, Macromolecular complexes from sheep and rabbit containing seven aminoacyl-tRNA synthetases. I. Species specificity of the polypeptide composition. Journal of Biological Chemistry. ,vol. 257, pp. 11041- 11048 ,(1982) , 10.1016/S0021-9258(18)33930-9
Marc MIRANDE, Daniel CORRE, Jean-Pierre WALLER, A complex from cultured Chinese hamster ovary cells containing nine aminoacyl-tRNA synthetases. Thermolabile leucyl-tRNA synthetase from the tsH1 mutant cell line is an integral component of this complex. FEBS Journal. ,vol. 147, pp. 281- 289 ,(1985) , 10.1111/J.1432-1033.1985.TB08748.X
Christian VADEBONCOEUR, Jacques LAPOINTE, Slow Diffusion of Glutamate and ATP‐Mg into High‐Molecular‐Weight Complexes Containing the Glutamyl‐tRNA Synthetase from Bovine Brain FEBS Journal. ,vol. 109, pp. 581- 587 ,(1980) , 10.1111/J.1432-1033.1980.TB04831.X
Vadeboncoeur Christian, Jacques Lapointe, Properties of the cytoplasmic glutamyl-tRNA synthetase in high molecular weight complexes from bovine brain. Brain Research. ,vol. 188, pp. 129- 138 ,(1980) , 10.1016/0006-8993(80)90562-4