Testing for Blood Group Immunological Reaction Without the Use of Anti-Human Globulin

摘要: A method testing for blood antigens (known as forward grouping) is presented wherein known antibodies are attached to a solid surface and the red cell sample immunological reaction centrifuged physically overcome natural repellent force between two cells (know in industry zeta potential) allow antigen antibody occur more rapidly. reverse grouping procedure utilizes synthetic or purified antigens, which directly surface. The then contacted with patient's serum occur. washed second labeled of concentration added an indicator. This performing major crossmatch that does not utilize anti-human immunoglobin. In alternative procedure, binder used attach membranes from donor recipient allowed undergo immune these on Antibody screening identification carried out by attaching carrying unknown solution will extent specific previously adhered present bind. Red particles indicator mechanism.