In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation

摘要: Left ventricular (LV) mass is an important descriptor of cardiac status that increases with normal aging and may be affected by a variety disease processes. There are currently limited noninvasive techniques permit accurate determination in vivo LV very small animals, such as the mouse, frequently used model for research. We sought to evaluate ability high-frequency (7.0 or 7.5 MHz), two-dimensional (2-D) guided M-mode echocardiography estimate mouse. Fifteen adult mice weighing 22-45 g were studied, including six young (2- 3-mo-old), two (12- 14-mo-old), seven senescent (18- 20-mo-old) animals. Resting heart rate varied up 450 beats/min. Anterior wall, inferior end-diastolic dimensions measured, echocardiographic (LVMe) was calculated using uncorrected cube approximation. Autopsy determined within 4 h examination. ranged from 88 211 mg. chamber included anterior wall (1.0 +/- 0.2 mm), (1.1 0.3 dimension (3.7 0.5 mm). good correlation between LVMe (x) autopsy (y):y = 0.96x - 7, r 0.94, standard error 18 mg, P < 0.001. This stronger than body weight (r 0.70) age 0.74), indexes which until now only correlates available this animal model. conclude that, despite rapid size mouse heart, these results demonstrate potential 2-D transthoracic assessment prove useful research on cardiomyopathies.