Tularaemia in European Brown Hares (Lepus europaeus) and Mountain Hares (Lepus timidus) Characterized by Histopathology and Immunohistochemistry: Organ Lesions and Suggestions of Routes of Infection and Shedding.

摘要: Tularaemia is an emerging zoonotic infectious disease caused by the bacterium Francisella tularensis. In Sweden, hares are considered to be key species in epidemiology of tularaemia. The aim this study was characterize pathology natural tularaemia infection European brown (EBHs) (Lepus europaeus) and mountain (MHs) timidus) order better understand presentation routes infection, body dissemination shedding F. During 2000-2013, 49 EBHs 37 MHs were diagnosed with Enlargement spleen seen 80% 62% MHs. Necrosis often obvious bone marrow, liver, lung spleen, but 30% had no lesions or minimal gross lesions. On microscopical examination tissues from 27 three MHs, necrosis majority samples lymph node adrenal glands common lungs brain meninges. Immunohistochemistry for spp. detected bacteria association inflammation. several cases, also found inside blood vessels, renal pelvis, lactating mammary glands, bronchioles skin, associated tick bites. Using quantitative polymerase chain reaction, two genotypes tularensis subsp. holarctica found; canSNP group B.6, all belonging subgroup B.7, B.12. There differences between genotypes. Our results indicate that urinary tract important Hunters may not aware risks contracting while handling hares, since infected do always show noticeable