Effect of strenuous exercise on platelet activation state and reactivity.
作者： A S Kestin , P A Ellis , M R Barnard , A Errichetti , B A Rosner
关键词:
摘要: BACKGROUNDIt has been hypothesized that platelets are activated, or made more activatible, by strenuous exercise and these changes may play a role in the genesis of exercise-induced coronary ischemia. Previous studies have yielded conflicting results but used assays (eg, platelet aggregation, plasma factor 4, beta-thromboglobulin) subject to methodological problems.METHODS AND RESULTSIn present study, whole blood flow cytometric method was study activation state reactivity 12 physically active sedentary individuals before after standardized treadmill testing. The peptide gly-pro-arg-pro (GPRP) included this assay prevent fibrin polymerization thus allowing measurement thrombin individual physiological milieu blood. A panel fluorescent-labeled monoclonal antibodies monitor activation-dependent surface c...
sci-hub.se 参考文章(80)
Charles Abrams, Sanford J Shattil, Immunological detection of activated platelets in clinical disorders. Thrombosis and Haemostasis. ,vol. 65, pp. 467- 473 ,(1991) , 10.1055/S-0038-1648173
SA Hamburger, RP McEver, GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood. ,vol. 75, pp. 550- 554 ,(1990) , 10.1182/BLOOD.V75.3.550.550
SJ Shattil, M Cunningham, JA Hoxie, Detection of activated platelets in whole blood using activation- dependent monoclonal antibodies and flow cytometry Blood. ,vol. 70, pp. 307- 315 ,(1987) , 10.1182/BLOOD.V70.1.307.307
AD Michelson, MR Barnard, Thrombin-induced changes in platelet membrane glycoproteins Ib, IX, and IIb-IIIa complex. Blood. ,vol. 70, pp. 1673- 1678 ,(1987) , 10.1182/BLOOD.V70.5.1673.1673
AD Michelson, PA Ellis, MR Barnard, GB Matic, AF Viles, AS Kestin, Downregulation of the platelet surface glycoprotein Ib-IX complex in whole blood stimulated by thrombin, adenosine diphosphate, or an in vivo wound. Blood. ,vol. 77, pp. 770- 779 ,(1991) , 10.1182/BLOOD.V77.4.770.770
P Hourdille, E Heilmann, R Combrie, J Winckler, KJ Clemetson, AT Nurden, Thrombin induces a rapid redistribution of glycoprotein Ib-IX complexes within the membrane systems of activated human platelets Blood. ,vol. 76, pp. 1503- 1513 ,(1990) , 10.1182/BLOOD.V76.8.1503.1503
HM Rinder, JL Bonan, CS Rinder, KA Ault, BR Smith, Dynamics of leukocyte-platelet adhesion in whole blood Blood. ,vol. 78, pp. 1730- 1737 ,(1991) , 10.1182/BLOOD.V78.7.1730.1730
CS Abrams, N Ellison, AZ Budzynski, SJ Shattil, Direct detection of activated platelets and platelet-derived microparticles in humans Blood. ,vol. 75, pp. 128- 138 ,(1990) , 10.1182/BLOOD.V75.1.128.128
S J Shattil, J A Hoxie, M Cunningham, L F Brass, Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. Journal of Biological Chemistry. ,vol. 260, pp. 11107- 11114 ,(1985) , 10.1016/S0021-9258(17)39154-8
BS Coller, EI Peerschke, LE Scudder, CA Sullivan, Studies with a murine monoclonal antibody that abolishes ristocetin- induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor Blood. ,vol. 61, pp. 99- 110 ,(1983) , 10.1182/BLOOD.V61.1.99.99