Reference Genes for Addressing Gene Expression of Bladder Cancer Cell Models under Hypoxia: A Step Towards Transcriptomic Studies

摘要: Highly aggressive, rapidly growing tumors contain significant areas of hypoxia or anoxia as a consequence inadequate and/or irregular blood supply. During oxygen deprivation, tumor cells withstand panoply adaptive responses, including shift towards anaerobic metabolism and the reprogramming transcriptome. One major mediators transcriptional hypoxic response is hypoxia-inducible factor 1 (HIF-1), whose stabilization under acts an oncogenic stimulus contributing to chemotherapy resistance, invasion metastasis. Gene expression analysis by qRT-PCR powerful tool for cancer phenotypic characterization. Nevertheless, undergo severe transcriptome remodeling.in deficit, precise identification reference genes poses challenge studies. Herein, we aim establish best studying effects on bladder cells. Accordingly, three cell lines (T24, 5637, HT1376) representative two distinct carcinogenesis pathways invasive (FGFR3/CCND1 E2F3/RB1) were used. Additionally, have explored most suitable control gene when addressing influence Deferoxamine Mesilate salt (DFX), iron chelator often used avoid proteasomal degradation HIF-1α, acting hypoxia-mimetic agent. Using bioinformatics tools (GeNorm NormFinder), elected B2M HPRT stable all experimental conditions out panel seven putative candidates (HPRT, ACTB, 18S, GAPDH, TBP, B2M, SDHA). These observations set molecular basis future studies effect particularly HIF-1α in