Phylogenetic analysis of rRNA methyltransferases, Erm and KsgA, as related to antibiotic resistance

摘要: It has long been speculated that erm and ksgA are related evolutionarily due to their sequence similarity analogous catalytic reactions. We performed a comprehensive phylogenetic analysis with extensive Erm KsgA/Dim1 sequences (Dim1 is the eukaryotic ortholog of KsgA). The tree provides insights into evolutionary history genes, showing early bifurcation Firmicutes Actinobacteria, suggesting origin current genes in pathogenic bacteria cannot be explained by recent horizontal gene transfer from antibiotic producers. On other hand, support commonly assumed relationships between common ancestry or descended preexisting ksgA, because unequivocally rooted insufficient signal long-branch attraction. indicates underwent frequent duplication, resulting anomalies atypical phenotypes. Several electronically annotated were recognized as candidates for new classes macrolide-lincosamide-streptogramin B-resistance determinants, sharing less than an 80% amino acid identity classes.