Circulating concentrations of fibroblast activation protein α in apparently healthy individuals and patients with acute coronary syndrome as assessed by sandwich ELISA.

摘要: Abstract Background Fibroblast activation protein α (FAP) is a membrane glycoprotein with dipeptidyl-peptidase and collagenase activity expressed in cancer, arthritis, atherosclerotic plaques. We hypothesized that FAP can be measured quantitatively the circulation provide prognostic information acute coronary syndrome (ACS). Methods assessed performance of commercially available ELISA pre-analytic characteristics marker. determined concentrations EDTA plasma samples from 101 apparently healthy blood donors 407 patients ACS. Patients were followed for 12months regarding all-cause mortality non-fatal myocardial infarction (MI). Results was stable at room temperature (for 1day) 4°C (3days) resistant to 3 freeze/thaw cycles. Recovery recombinant human ranged 78 103% serial dilutions spiked resulted measurements within 91 120% expected values. ACS had lower compared [median (25th–75th percentiles): 84 (69–101)ng/mL vs. 108 (87–124)ng/mL, P Conclusions Our study first associate prognosis The favorable will facilitate future studies marker other disease settings associated altered expression.