Effect of subconjuctival and intraocular bevacizumab injection on angiogenic gene expression levels in a mouse model of corneal neovascularization

摘要: Purpose: This study sought to characterize the expression of angiogenesis-related genes in a mouse model corneal neovascularization, either untreated or after treatment with single injection bevacizumab by three different routes. In addition, effectiveness was compared rabbit model. Methods: A chemical burn induced mid-cornea right eye 119 mice; 56 them were and 63 bevacizumab-treated. Neovascularization evaluated 2, 4, 8, 10, 14 days later using digital photos, angiography India ink perfusion. The relative area new blood vessels analyzed slit-lamp examination vivo on histological flat-mount sections. levels gene involved angiogenic process vascular endothelial growth factor [VEGF], insulin-like factor-1 [IGF-1], pigment epithelium derived [PEDF], macrophage-inflammatory protein-2 [MIP-2]) measured real-time polymerase chain reaction. Six rabbits underwent same injury treatment, response Results: first observed two injury. affected section increased from 11.24% (±7.0) 47.42% (±25.4) day 8 50.62% (±24.7) 10. mice treated bevacizumab, neovascularization significantly lower at peak time points (p<0.005): 24.90% (±21.8) 28.29% (±20.9) Spontaneous regression both groups, 26.98% (±19.9) 10.97% (±10.8) bevacizumab-treated (p<0.005). Rabbits also showed 8-10, significant following intracameral injection. mice, intraocular (intravitreal, intracameral) more effective than subconjuctival VEGF upregulated but slightly less mice. PEDF decreased group, peaked (above baseline) days, it had already 8. IGF-1 early model; there only slight change group increase group. MIP-2 groups stage returned baseline 14. Conclusions: Bevacizumab partially inhibits progressive damage Treatment is when administered via subconjunctival route. clinical findings are compatible angiographic histologic data supported molecular analysis showing partial proangiogenic genes. mechanisms inflammation warrant further exploration. These may have important therapeutic implications setting.