Cloning and characterization of human astrovirus immunoreactive epitopes.

摘要: Abstract We report the cloning of antigenic, protein-coding regions human astrovirus serotype 1 that appear to be common most, if not all, serotypes astrovirus. Screening lambda gt11 libraries identified three different but overlapping clones (A43, A35, and A1) one independent clone (A14) reacted with serum from a rabbit repeatedly immunized purified particles its preimmunization serum. These were shown specific. Of note, radiolabeled probe representing immunoreactive A43-A35-A1 hybridized exclusively 7.2-kb genomic RNA, while A14-specific both 2.8-kb subgenomic RNAs. This suggests epitopes, selected by antiserum particles, are encoded RNA as well other RNA.