Products of the lipoxygenase pathway in human natural killer cell cytotoxicity

摘要: As earlier data suggested the importance of lipoxygenase activation for expression human NK cell cytotoxicity, four different inhibitors were tested suppression natural killer (NK) lysis. All found active at nontoxic concentrations with 50% inhibition approximately 15 microM nordihydroguaiaretic acid (NDGA). lysis could be reconstituted to NDGA-suppressed cells leukotriene B4 (LTB4), all-trans isomers 6-trans-LTB4 and 12-epi-6-trans-LTB4, 20-COOH-LTB4. LTB4 reconstitution was best in concentration range 1-100 pM near control levels both higher lower concentrations. Herpesvirus Ateles-transformed T also inhibited by NDGA. These indicate that activity is required several LTB4-related products can restore cells; they suggest pathway present population.