Endothelial cell compatibility testing of three different Pellethanes.

摘要: There is a need for viable small diameter vascular grafts, the luminal surface of which could be seeded by endothelial cells (ECs) to prevent thrombosis. In order select candidates EC seeding before implantation, in vitro cytocompatibility three different Pellethanes (polyetherurethanes) using human ECs was investigated. The methodology included two stages depending on either direct contact between and materials or material extracts, obtained under standardized conditions. By latter method, we observed cytotoxic effect cell growth with 2363-55 D Pellethane extract at 50% (v/v) concentration nutrient medium, likely provoked leachables correlated lowest levels tPA, PAI1, vWF antigens supernatants. former studied attachment growth. Morphology classical means completed scintigraphy microautoradiography after 111Indium-labeling monolayer. Differentiation determined release antigen measurement activity (multimeric organization) thrombin stimulation. Despite an inhibition proliferation both 55 75 types (compared control), functional monolayer D. best support endothelization.