Markers for trans-Golgi Membranes and the Intermediate Compartment Localize to Induced Membranes with Distinct Replication Functions in Flavivirus-Infected Cells

摘要: Replication of the flavivirus Kunjin virus is associated with virus-induced membrane structures within cytoplasm infected cells; these membranes appear as packets vesicles sites viral RNA synthesis and convoluted (CM) paracrystalline arrays (PC) containing components virus-specified protease (E. G. Westaway, J. M. Mackenzie, T. Kenney, K. Jones, A. Khromykh, Virol. 71:6650-6661, 1997). To determine cellular origins structures, we compared immunolabelling patterns several cell markers in relation to by immunofluorescence immunoelectron microscopy. A marker for trans-Golgi network, 1,4-galactosyltransferase (GalT), was redistributed large foci virus-infected cells, partially coincident immunofluorescent putative synthesis. As determined microscopy, induced vesicle contained GalT, whereas CM PC a specific protein intermediate compartment (ERGIC53). further indicator role organelles their biogenesis observation that Golgi apparatus-disrupting agent brefeldin prevented development if added before end latent period but once formed, were resistant dispersion. Reticulum emanating from also labelled rough endoplasmic reticulum anti-protein disulfide isomerase obviously during infection. This first report identifying apparent sources flavivirus-induced involved events replication.