Capillary electrophoresis of diuretics and probenecid in methanol

摘要: Actual mobilities and dissociation constants of six diuretics (benzthiazide, bumetanide, ethacrynic acid, furosemide, hydrochlorothiazide chlorothiazide) probenecid were investigated in methanol by capillary zone electrophoresis (CZE). The actual derived from the dependence effective analytes on pH(*) methanolic background electrolyte (BGE) solution. measurement was carried out mainly a pressure-mediated (CE) method. For comparison, parallel measurements for two conventional approach, without pressure. pK(a)(*) values calculated non-linear curve fitting to measured mobility values. difference between pK(a) water about 5 units loop (furosemide, acid) around 3-4 thiazide diuretics. Knowledge ionisation behaviour compounds paves way wider use as solvent electrophoresis. Moreover, is demonstrated current study, calculation facilitates optimisation pH conditions separation, thereby applications can be expanded, combination with electrospray mass spectrometry becomes easier.