Improved production of the indole alkaloid canthin-6-one from cell suspension culture of Brucea javanica (L.) Merr.

摘要: In an attempt to increase the productivity of pharmaceutical compound canthin-6-one from cell suspension culture Brucea javanica, a high density and improved conditions have been investigated with focus on stages growth. The highest yield canthin-6-one, 26.72 mg g -l dry cell, was produced intracellular in 50 L -1 mass. established suspensions were harvested at age 40 days placed into MS medium containing 1.0 L-1 2,4-D, NAA, 0.1 Kinetin 10 tryptophan for 33-40 days, approximately when cultures their late stationary phase Addition 20 did not show significant difference production (p>0.05). Higher than showed adverse effect concentration canthin-6- one alkaloid (p=0.01). Qualitative analysis chloroform-extracts dried cells revealed spots TLC; identity by comparison its Rf value (Rf=0.37), colour reaction Dragendorff reagent ammonium sulphate behaviour UV authentic sample. Quantification using TLC- densitometry scanner response different as precursor concentration-dependent response. weight this study, establishes methodology procedure compared those previously reported offers opportunity industrial level.