Polymerase chain reaction using 16S rRNA gene sequences distinguishes the two biovars of Ureaplasma urealyticum.

摘要: Several fundamental phenotypic and genotypic differences have separated strains of the genital mycoplasma Ureaplasma urealyticum into two clusters or biovars. However, lack an easily performed unambiguous test to discriminate between them has hampered investigation relationship these biovars disease. We determined 16S rRNA nucleotide sequence U. 27, serovar 3 standard representative parvo biovar (serovars 1, 3, 6, 14). This was compared with published T960, which is type strain 8 T960 composed 10 intervening serovars. Homology sequences 98.8%; were exploited provide primers for biovar-specific polymerase chain reactions (PCRs). The results placed all 14 correct biovar. PCRs also applied cloned noncloned isolates that had been serotyped earlier. For 16 them, we deduced their from serotyping data then confirmed by PCR. One unpredictable isolate one nonserotypeable classified as Thus, developed a method biotyping applicable both laboratory-adapted wild-type appropriate testing large numbers clinical isolates. amplification PCR protocol DNAs ureaplasmas animals certain Mycoplasma species suggested diverged mainstream evolution this clade.