A simple approach for the preparation of mature human relaxin-3.

摘要: Relaxin-3 (also known as INSL7) is the most recently identified member of insulin-like family. It predominantly expressed in nucleus incertus brain and involved control stress response, food intake, reproduction. In present work, we have established a simple approach for preparation mature human relaxin-3 peptide. We first designed recombinantly single-chain precursor E. coli cells. After purification by immobilized metal ion affinity chromatography, refolding vitro through disulfide reshuffling, digestion endoproteinase Asp-N, was obtained high yield at low cost. Peptide mapping circular dichroism spectroscopy studies suggested that recombinant adopted an fold with expected linkages. The fully active both RXFP3 binding activation assays. activity very low, suggesting free C-terminus B-chain necessary receptor-binding relaxin-3. Our work provides highly efficient well its analogues functional structural analyses.