Role of endothelial cell-substrate contact area and fibronectin-receptor affinity in cell adhesion to HEMA/EMA copolymers.
作者： Jeffrey S. Burmeister , Valerie Z. McKinney , William M. Reichert , George A. Truskey
DOI: 10.1002/(SICI)1097-4636(19991215)47:4<577::AID-JBM15>3.0.CO;2-S
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摘要: The objective of this study was to examine the effect substrate hydrophobicity on cell-substrate contact area and affinity between adsorbed fibronectin (Fn) its receptor. Homo- copolymer films hydrophobic ethyl methacrylate (EMA) hydrophilic hydroxyethyl (HEMA) were spun-cast onto glass slides. Bovine aortic endothelial cells (BAEC) plated for 2 h in serum-free medium polymers preadsorbed with Fn. Cells fixed, labeled, examined by total internal reflection fluorescence microscopy (TIRFM) determine topography basal surface as a function distance from substrate. Phase contrast used projected adherent cells. cumulative greatest attached surfaces prepared 0% HEMA lowest highest content. An equilibrium adhesion model these data together critical force detachment Fn density (Burmeister et al., J Biomed Mater Res 1996;30:13-22) receptor bond strength. per decreased increasing These results indicate that differences strength cell are influenced conformation proteins.
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