Development of a disposable ethanol biosensor based on a chemically modified screen-printed electrode coated with alcohol oxidase for the analysis of beer

作者: Mohammed Boujtita , John P Hart , Robin Pittson

DOI: 10.1016/S0956-5663(00)00075-0

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摘要: A disposable amperometric biosensor for the measurement of ethanol has been developed. It comprises a screen-printed carbon electrode doped with 5% cobalt phthalocyanine (CoPC-SPCE), and coated alcohol oxidase; permselective membrane on surface acts as barrier to interferents. The is based signal produced by H2O2, product enzymatic reaction. Optimisation studies were performed using amperometry in stirred solution magnitude was found be dependent pH, enzyme loading, type applied potential. same technique used evaluate determination samples. results obtained compared well manufacturers specifications. In order test possibility devices field, chronoamperometry also determine beer precision recovery data again indicated that should give reliable under conditions described.

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