Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System

摘要: The purpose of this research is to establish a process irradiating mice using the Gamma Knife as versatile system for small animal irradiation and validate accurate intracranial extracranial dose delivery system. A stereotactic immobilization device was developed animals head frame allowing isocentric delivery. Intercranial positional reproducibility reference point from primary verified on an additional mouse. Extracranial mouse aorta 3 mice. Accurate validated film thermoluminescent dosimeter measurements with solid water phantom. plans were irradiate targets. Mice irradiated validating successful targeted radiation Intramouse variability right mandible across 10 micro-computed tomography scans 0.65 ± 0.48 mm. Intermouse 2 at same 0.76 0.46 accuracy 0.67 0.29 mm 1.01 0.43 in coronal sagittal planes, respectively. planned delivered phantom Gy 50% isodose measured 2.9 0.3 Gy. phosphorylated form member X histone family H2A (γH2AX) staining brain demonstrated adjacent tissue sparing. In conclusion, our preclinical studies able accurately deliver focal experiments studying radiation.