Hydrolysis of Isolate Soybean Protein Using Subcritical Water

摘要: Hydrolysis of isolate soybean protein (ISP) using subcritical water (SCW) was conducted to study the feasibility for producing hydrolyzate. SCW hydrolysis SPI suspension (5-15%) in an electrically heated batch reactor (2 L). The effects temperature (230 270 o C) and holding time (10 50 min) on degree (DH) production amino acids were studied by surface response method. DH determined derivatizing hydrolyzates with ortho-phthalaldehyde (OPA) solution. It confirmed that reaction affected hydrothermolysis protein. However, less effective acid yield when higher than 230 C. In order achieve optimal yields exceeding 43%, should be within range between 256 268 C from 29 41 min, respectively. A maximum estimated 43.5% obtained at 35 min.